Trichomonas vaginalis is a parasitic protist that infects the human urogenital tract. During the infection, trichomonads adhere to the host mucosa, acquire nutrients from the vaginal/prostate environment, and release small extracellular vesicles (sEVs) that contribute to trichomonad adherence and modulate host-parasite communication. Approximately 40–70% of T. vaginalis strains harbor a double-stranded RNA virus called Trichomonasvirus (TVV). Accumulated evidence suggests that the endosymbiotic TVV may play a role in the pathogenesis and drug susceptibility of T. vaginalis. However, how TVV interacts with host cells is still unclear. This investigation will employ second and third-generation sequencing technologies to examine the TVV-host interactome. Participants are anticipated to spend 1-2 months in the laboratory, gaining practical skills in conducting high-throughput sequencing experiments and acquiring proficiency in data analysis through a bioinformatics approach.