Dendritic cells (DCs), including plasmacytoid DC (pDC) and conventional DC (cDC), are key immune cells linking innate and adaptive immunity. However, their developmental programs and functions during inflammation and infection are not completely understood. In this project, we will take advantage of lentivirus-mediated knockdown and CRISPR-Cas9 knockout techniques to study the origins and signals controlling their homeostasis and functionality. Participants will receive training in RT-QPCR, viral transduction, flow cytometry, and handling of experimental animals. Maximum number of participants is 2 people based on the quality of the application form. Each participant in this project will receive a scholarship of NTD 15000-25000/mo for at least 3 months and up to 5 months.