The main execution plan is as follows, but it is not limited to this. Feel free to discuss and implement any experimental ideas related to microbiology that you may have. Over the past few years, Shiitake mushrooms (Lentinus edodes) have garnered significant global attention, cementing their position as the second most consumed mushroom worldwide. Despite their increasing popularity, the shiitake mushroom industry confronts a range of challenges, notably from mycoviruses that jeopardize their cultivation and yield. While mycovirus infections have been detected in cultivated shiitake mushrooms, their precise impact on the sustainability of the industry remains a critical concern, especially in key production regions like Taiwan. To counter the detrimental effects of mycoviruses, it is essential to comprehensively grasp their distribution and diversity within cultivated mushrooms. Consequently, a comprehensive investigation is planned to assess the prevalence of mycoviruses in shiitake mushrooms cultivated in mushroom farm in Taiwan, yielding crucial insights for the development of targeted containment strategies. Additionally, the project will concentrate on refining the composition of mushroom bags to minimize the risk of virus infections, safeguarding overall productivity on mushroom farms. Another pressing challenge for the shiitake mushroom industry revolves around preserving various mushroom strains through cryopreservation. The susceptibility of these strains to virus infections, coupled with the potential impact of climate change, underscores the urgency of identifying effective cryopreservation methods. Innovations in cryopreserving mushroom filaments remain elusive, necessitating the exploration of advanced hybrid hydrogel systems integrating natural polymers like bacterial cellulose, chitin, and galactoxyloglucan. Through a comprehensive study of the synthesis process and efficacy evaluation of these hydrogels in preserving shiitake mushroom strains, the project endeavors to establish a reliable and sustainable long-term cryopreservation approach. Given the increasing integration of global food trade, the accurate identification of product origins has become pivotal. However, only a few studies have successfully differentiated the source of shiitake mushroom strains consumed in Taiwan, primarily relying on sensory assessments based on the shiitake fruiting body's morphological features. Hence, the project is exploring molecular-genetic markers such as RAPD and SCAR assays to distinguish various L. edodes strains, with potential applications in mushroom breeding and enhancing strain characteristics. Furthermore, the shiitake mushroom industry must address global concerns regarding environmental sustainability and plastic waste. Given the prevalent use of plastic bags in cultivation, the generation of waste from spent mushroom substrates has become a critical issue. To tackle this, the project proposes a comprehensive circular economy approach, repurposing the spent mushroom substrate to minimize environmental impact. Specifically, the project aims to develop an eco-friendly and cost-effective method for synthesizing silver nanoparticles (AgNPs) from residual shiitake mycelium extracted from spent mushroom substrates. Leveraging the antimicrobial properties of these nanoparticles, the project seeks to create an effective disinfectant solution for mushroom farming, promoting food safety and sustainability within the industry. The mushroom company (元益多生物科技股份有限公司) will host workshops and training sessions focusing on advanced strain preservation methods, techniques for remedying mushroom strain degradation, precise environmental control in mushroom cultivation, efficient management of environmental facilities, high-pressure boiler sterilization procedures, automated bottling, and field experiments to evaluate the antibacterial properties of the synthesized silver nanoparticles. Through active participation in laboratory research and practical fieldwork at the mushroom company, the project aims to equip interns with valuable skills and insights, nurturing the next generation of experts in the field of mushroom cultivation and sustainable agriculture. During the internship, a scholarship of approximately 15,000 per month will be provided, with the actual amount based on the interview results. The internship period is expected to be at least six months.