Objectives of this Program: We have planned to develop “Next generation enzymes-oriented materials and enzyme-incorporated nanomaterials for cancer and biomarker detection technologies” Additionally, we aim to increase international student enrolment in the Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, by promoting various interdisciplinary research directions in advanced biosensing, nanotechnology, and biomedical engineering.

Research Plan: In this research, an advanced electrochemical sensor was designed for detecting cancer biomarkers and clinically relevant analytes by harnessing the capabilities of innovative enzymes and enzyme-incorporated nanomaterials. Special emphasis was placed on the use of surface-engineered platforms such as screen-printed carbon electrodes (SPCEs) and laser-induced graphene (LIGs), which offer adaptable and highly conductive surfaces for effective enzyme immobilisation and biosensor fabrication. To construct the sensing interface, biocompatible substrates like chitosan, reduced graphene oxide (rGO), and gold nanoparticles were selected for their outstanding ability to stabilise enzymes and facilitate rapid electron exchange. Hydrothermal synthesis and detailed physical characterisation ensured these materials not only provided an increased density of catalytic sites but also boosted sensor sensitivity.

Electrochemical performance was meticulously evaluated through cyclic voltammetry and differential pulse voltammetry, optimising conditions such as pH, scan rate, and analyte concentration to achieve reliable biomarker detection, even at trace levels within biological fluids. The natural affinity of chitosan for enzyme binding, combined with the high surface area and conductivity of rGO and gold nanoparticles, greatly improved biochemical recognition and electronic response. Moreover, the surface features and functional groups inherent to these materials promoted fast electron transfer kinetics and enhanced catalytic activity.

The overall fabrication approach leveraged scalable methods, including drop-casting and inkjet printing, guaranteeing consistent and reproducible sensor architecture. This strategy yields an accessible and robust electrochemical biosensor platform, ideally suited for early cancer detection and biomarker analysis, with the potential to underpin future point-of-care diagnostics and improve clinical outcomes.

Expected outcomes: We aim to attract undergraduate, master’s, and PhD students to engage in innovative research activities at National Taipei University of Technology. The programme will serve as a platform for exploring Taiwan’s academic excellence, culture, and technological advancement, while providing advanced training and career development opportunities. Through international initiatives such as TEEP and collaborations with leading universities, students will gain global exposure and enhance their employability. As Chairman of the Department of Chemical Engineering and Biotechnology, NTUT. I will promote international partnerships and industrial collaborations in biomedical applications, showcasing Taiwan’s culture and innovation to the world. Participation will foster intercultural exchange, improve communication skills, and contribute to Taiwan’s visibility and growth in global science and technology.

Collaboration Countries: Malaysia, India, Indonesia, Thailand, and Philippines.

We plan to hire more master’s and PhD students to enhance collaborations and product development. Funding will be provided in accordance with TEEP department and MOE regulations, with monthly funding ranging from 15,000 to 20,000 NTD. Some funding will be covered from school projects for their research. The majority of research support will be covered by National Taipei University of Technology projects, including chemical apparatus, instruments, and characterization facilities. Additionally, school projects will cover the APC charges for open-access publications.