In this project, we will provide 15,000 NTD per month stipend for 6 months. The expenses will need to be covered flight and dormitory costs within this amount. NPUST will provide the accommodation for the internship student in NPUST campus.

In the first 3 months, the NPUST dormitory costs will be waived. When discussing renewable energy, hydrogen emerges as an immensely promising source. Over recent years, biohydrogen production technology has witnessed continuous expansion. Dark fermentation and photo-fermentation currently serve as the predominant methods for hydrogen generation. Dark fermentation utilizes heterotrophic bacteria to decompose carbohydrates under anaerobic conditions, yielding fatty acids, alcohols, and hydrogen. Conversely, photo-fermentation involves phototrophic bacteria that convert carbohydrates into ADP and hydrogen in a light-induced setting. The amalgamation of these techniques holds potential to elevate hydrogen production and enhance energy conversion efficiency. This study endeavors to amalgamate dark fermentation and photo-fermentation technologies by immobilizing hydrogen-producing microorganisms under controlled conditions. It aims to optimize immobilization methods and selected carbon sources.

Additionally, molecular biology techniques will be employed to scrutinize alterations in microbial metabolic pathways. Gene expression analysis, enzyme activity assays, and elemental analysis will track the variations in hydrogen-producing microorganisms' performance, establishing optimal immobilization methods and continuous-flow reaction systems. This research direction significantly contributes to the sustainable development of hydrogen energy, offering more efficient and sustainable solutions for hydrogen production. Future research will delve into microbial growth behavior under immobilization conditions, focusing on how immobilization influences their metabolic characteristics to boost hydrogen yield and capacity. Continuous optimization of operational conditions for immobilization and continuous-flow reaction systems will be pursued to maximize efficiency and stability in hydrogen production.

In conclusion, advancements in this research direction hold farreaching implications for the sustainable future of hydrogen energy. Through the integration of dark fermentation and photo-fermentation technologies and investigations into optimized immobilization methods and variations in microbial metabolic pathways, our aim is to develop more competitive and sustainable approaches to hydrogen energy production, substantially contributing to the clean transition of future energy.